Cell culture flasks are specially designed for the rapid growth and multiplication of microbial, insect, or mammalian cells.
Basic Cell Culture & Expansion: Large-scale cell propagation (e.g., adherent or suspension cell lines like HEK293, CHO, Vero). Long-term maintenance of cell stocks for experiments or cryopreservation.
Requirements for Adherent Cell Culture: Surface treatment: TC-treated (tissue culture-treated) polystyrene for optimal cell attachment. Growth area: Available in sizes from 25 cm² (T-25) to 225 cm² (T-225) for scaling up cultures. Vented caps: Allow gas exchange (CO₂/O₂) while minimizing contamination risk.
Suspension Cell Culture: Non-treated surfaces: Prevent cell attachment (e.g., for hybridoma or insect cell cultures).
Cryopreservation & Storage: Cryo-resistant materials: Withstand -80°C to 37°C for freezing/thawing cells in situ. Erlenmeyer-style flasks: With baffled bases for improved aeration in shaker incubators.